 ผลงานตีพิมพ์ในวารสารวิชาการAntibiotic use in mandarin production (Citrus reticulata Blanco) in major mandarin producing areas in Thailand: A survey assessmentผู้แต่ง: Sunicha Chanvatik,  Dr.Siriporn Donnua, Assistant Professor , Angkana Lekagul, Wanwisa Kaewkhankhaeng, Vuthiphan Vongmongkol, Pornpimon Athipunyakom, Saenchai Khamlar, Maitree Prommintara, Viroj Tangcharoensathien, วารสาร: |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Isolation and Identification of Mycorrhizal Fungi from Eleven Terrestrial Orchids) ผู้เขียน:Pornpimon Athipunyakom, ดร.เลขา มาโนช, รองศาสตราจารย์ , นางจิตราพรรณ พิลึก, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractMycorrhizal fungi were isolated from eleven terrestrial orchid species, namely Calanthe rubens, Calanthe rosea, Cymbidium sinense, Cymbidium tracyanum, Goodyera procera, Ludisia discolor, Paphiopedilum concolor, P. exul, P. godefroyae, P. niveum and P. villosum. Healthy roots of orchid hosts were collected from various parts of the country. Isolation of mycorrhizal fungi from pelotons was carried out using a modification of Masuhara and Katsuya method. Identification was based on morphological characteristics. Nuclei were stained with safranin O using Bandoni’s method. Seven genera and fourteen species of mycorrhizal fungi were identified: Ceratorhiza cerealis, C. goodyerae-repentis, C. pernacatena, C. ramicola, Ceratorhiza sp., Epulorhiza calendulina, E. repens, Rhizoctonia globularis, Sistotrema sp., Trichosporiella multisporum, Tulasnella sp. Waitea circinata and two Rhizoctonia species. Nuclear staining revealed that all strains were binucleate except for W. circinata which was multinucleate. Pure cultures were maintained on PDA slant and liquid paraffin at the Culture Collection, Department of Plant Pathology, Kasetsart University. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Mycorrhizal Fungi from Spathoglottis plicata and the Use of these
Fungi to Germinate Seeds of S. plicata in vitro) ผู้เขียน:Pornpimon Athipunyakom, ดร.เลขา มาโนช, รองศาสตราจารย์, นางจิตราพรรณ พิลึก, รองศาสตราจารย์, Suparp Artjariyasripong, Somwong Tragulrung สื่อสิ่งพิมพ์:pdf AbstractHealthy roots from mature plants of terrestrial orchid, Spathoglottis plicata (Blume.) collected
from Chiang Mai, Chanthaburi, Nakhon Ratchasima and Bangkok were used to isolate mycorrhizal fungi.
A modification of Masuhara and Katsuya’s method was employed to isolate these fungi from the peloton.
Identification was based on morphological characteristics. Nuclei were stained with safranin O using
Bandoni’s method revealing that all isolates were binucleate. Three genera and four species of
mycorrhizal fungi were identified: Epulorhiza repens, Rhizoctonia globularis, Rhizoctonia sp. and
Sabacina sp. (Epulorhiza sp. anamorph). In vitro, symbiotic seed germination and development on seeds
of S. plicata were tested using Epulorhiza repens and Rhizoctonia globularis. It was found that 99.2%
of seeds inoculated with Epulorhiza repens and 96.3% of seeds inoculated with Rhizoctonia globularis
germinated within 21 days, whereas only 15% of seeds incubated in the absence of fungi germinated.
About 8.1% of seeds inoculated with Epulorhiza repens and 1.7% of seeds inoculated with Rhizoctonia
globularis initiated leaves after 60 days of incubation, whereas in the absence of fungi they did not
develop into seedlings and mortality gradually occurred. The seedlings became more mature and ready
to be planted after 127 days. |
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